Composite

Part:BBa_K3544105

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-22)


NLS-yeGFP-CRISPR(Csy4)-DsRed

To verify whether the proteins translated from sequences followed by NLS and CRISPR (Csy4) can be located in the nucleus and cytoplasm, respectively

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1387
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1387
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696


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Categories
Parameters
None